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CEH is associated with enhanced intestinal mucosal repair and activation of the lactate–Paneth cell–Wnt axis in adult mice. (A) Levels of lactate in the intestinal tract (n = 6); (B) Western blot analysis of LYZ1 and <t>Wnt3A;</t> (C, D) LYZ1 and Wnt3A protein quantification (n = 6). Data are mean ± SD. Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001(n=3). (E) LYZ1 + Wnt3A + immunofluorescence (20×, scale bar = 50 μm); (F) Mean fluorescence intensity (MFI) of LYZ1 + Wnt3A + (n = 6); (G) Positive cells areas for LYZ1 + Wnt3A + (n = 6). Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001.
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CEH is associated with enhanced intestinal mucosal repair and activation of the lactate–Paneth cell–Wnt axis in adult mice. (A) Levels of lactate in the intestinal tract (n = 6); (B) Western blot analysis of LYZ1 and Wnt3A; (C, D) LYZ1 and Wnt3A protein quantification (n = 6). Data are mean ± SD. Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001(n=3). (E) LYZ1 + Wnt3A + immunofluorescence (20×, scale bar = 50 μm); (F) Mean fluorescence intensity (MFI) of LYZ1 + Wnt3A + (n = 6); (G) Positive cells areas for LYZ1 + Wnt3A + (n = 6). Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001.

Journal: Frontiers in Immunology

Article Title: Casein hydrolysate promotes intestinal repair in adult mice with Salmonella enteritis through a lactate-GPR81-Wnt3A axis in intestinal stem cell niche

doi: 10.3389/fimmu.2026.1817158

Figure Lengend Snippet: CEH is associated with enhanced intestinal mucosal repair and activation of the lactate–Paneth cell–Wnt axis in adult mice. (A) Levels of lactate in the intestinal tract (n = 6); (B) Western blot analysis of LYZ1 and Wnt3A; (C, D) LYZ1 and Wnt3A protein quantification (n = 6). Data are mean ± SD. Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001(n=3). (E) LYZ1 + Wnt3A + immunofluorescence (20×, scale bar = 50 μm); (F) Mean fluorescence intensity (MFI) of LYZ1 + Wnt3A + (n = 6); (G) Positive cells areas for LYZ1 + Wnt3A + (n = 6). Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001.

Article Snippet: Membranes were blocked with 5% non−fat milk in TBST for 2 h at room temperature, then incubated overnight at 4 °C with primary antibodies against Wnt3A (A25322, Abclonal, 1:700), LYZ1 (518083, Santa cruz, 1:1000), and β−actin (internal control).

Techniques: Activation Assay, Western Blot, Immunofluorescence, Fluorescence

CEH improves intestinal epithelial regeneration, barrier function, and immune homeostasis in Salmonella-infected adult mice. (A, B) Analysis of LYZ1 + Wnt3A + immunofluorescence staining outcomes (20×, scale bar = 50 μm, n=6); (C, D) Analysis of LYZ1 + GPR81 + immunofluorescence staining outcomes (20×, scale bar = 50 μm, n=6); (E, F) Analysis of LGR5 + Ki67 + immunofluorescence staining outcomes (20×, scale bar = 50 μm, n=6); (G, H) Analysis of E-cadherin + flow cytometry staining results (n=6); (I, J) Analysis of CD45 + flow cytometry staining results (n=6); (K, L) Analysis of CD24 + LGR5 + flow cytometry staining results (n=6); (M) TNF-α level in adult mice (n = 6); (N) IL-10 level in adult mice (n = 6). Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001.

Journal: Frontiers in Immunology

Article Title: Casein hydrolysate promotes intestinal repair in adult mice with Salmonella enteritis through a lactate-GPR81-Wnt3A axis in intestinal stem cell niche

doi: 10.3389/fimmu.2026.1817158

Figure Lengend Snippet: CEH improves intestinal epithelial regeneration, barrier function, and immune homeostasis in Salmonella-infected adult mice. (A, B) Analysis of LYZ1 + Wnt3A + immunofluorescence staining outcomes (20×, scale bar = 50 μm, n=6); (C, D) Analysis of LYZ1 + GPR81 + immunofluorescence staining outcomes (20×, scale bar = 50 μm, n=6); (E, F) Analysis of LGR5 + Ki67 + immunofluorescence staining outcomes (20×, scale bar = 50 μm, n=6); (G, H) Analysis of E-cadherin + flow cytometry staining results (n=6); (I, J) Analysis of CD45 + flow cytometry staining results (n=6); (K, L) Analysis of CD24 + LGR5 + flow cytometry staining results (n=6); (M) TNF-α level in adult mice (n = 6); (N) IL-10 level in adult mice (n = 6). Significance was accepted at *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001.

Article Snippet: Membranes were blocked with 5% non−fat milk in TBST for 2 h at room temperature, then incubated overnight at 4 °C with primary antibodies against Wnt3A (A25322, Abclonal, 1:700), LYZ1 (518083, Santa cruz, 1:1000), and β−actin (internal control).

Techniques: Infection, Immunofluorescence, Staining, Flow Cytometry